Designed by: Yiran Wu Group: iGEM14_SCUT-China (2014-10-07)
loading module+module(1+2)
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1827 Illegal BamHI site found at 6372 Illegal BamHI site found at 9222
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 507 Illegal NgoMIV site found at 1990 Illegal NgoMIV site found at 2362 Illegal NgoMIV site found at 2395 Illegal NgoMIV site found at 2868 Illegal NgoMIV site found at 3577 Illegal NgoMIV site found at 4450 Illegal AgeI site found at 318 Illegal AgeI site found at 1680 Illegal AgeI site found at 2026 Illegal AgeI site found at 2605 Illegal AgeI site found at 4326 Illegal AgeI site found at 4396 Illegal AgeI site found at 5023
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1432 Illegal BsaI.rc site found at 3862 Illegal SapI.rc site found at 2697
Design Notes
Source
Saccharopolyspora erythraea
References
[1]Cane, David E. Programming of erythromycin biosynthesis by a modular polyketide synthase [J]. Journal of Biological Chemistry, 2010, 285.36: 27517-27523.
[2]Komaki, Hisayuki, et al. Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species [J]. BMC genomics 15.1 (2014): 323.
[3]Pfeifer, Blaine A., et al. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli [J]. Science, 2001, 291.5509: 1790-1792.
[4]Tae, Hongseok, Jae Kyung Sohng, and Kiejung Park. Development of an analysis program of type I polyketide synthase gene clusters using homology search and profile hidden Markov model [J]. Journal of microbiology and biotechnology, 2009, 19.2: 140-146.
[5]Cortes, Jesus, et al. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea [J]. 1990: 176-178.
[6]Khosla, Chaitan, Shiven Kapur, and David E. Cane. Revisiting the modularity of modular polyketide synthases [J]. Current opinion in chemical biology, 2009, 13.2: 135-143.
[7]Menzella, Hugo G., et al. Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster [J]. Journal of Industrial Microbiology and Biotechnology,2006, 33.1: 22-28.
[8]Oliynyk, Markiyan, et al. A hybrid modular polyketide synthase obtained by domain swapping [J]. Chemistry & biology,1996, 3.10: 833-839.
[9]Lau, Janice, David E. Cane, and Chaitan Khosla. Substrate specificity of the loading didomain of the erythromycin polyketide synthase [J]. Biochemistry, 2000, 39.34: 10514-10520.
[10]Nowak-Thompson, Brian, et al. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5 [J]. Journal of bacteriology, 1999, 181.7: 2166-2174.
[11]Caffrey, Patrick, et al. Amphotericin biosynthesis in Streptomyces nodosus deductions from analysis of polyketide synthase and late genes [J]. Chemistry & biology,2001, 8.7: 713-723. [12]Dunn, Briana J., et al. Comparative analysis of the substrate specificity of trans-versus cis-acyltransferases of assembly line polyketide synthases [J]. Biochemistry,2014.
[13]Jiang, Ming, and Blaine A. Pfeifer. Metabolic and pathway engineering to influence native and altered erythromycin production through E. Coli [J]. Metabolic engineering, 2013, 19: 42-49. [14]Chen, Xianzhong, et al. Metabolic engineering of Escherichia coli: A sustainable industrial platform for bio-based chemical production [J]. Biotechnology advances, 2013, 31.8: 1200-1223.
